Mouse Samples

1. Samples were collected from left ventricle of adult mice treated with AngII and PE to induce heart failure.

2. Treatment lasts for 3 and 10 days. Day 3 samples were sequenced at July 2022, Day 10 samples were sequenced at Feb and July, 2022.

3. Contain both female and male.

Treatment Sex Feb 2022 (D10) July 2022 (D10) July 2022 (D3)
CTL Female B2, B3, H3, H6 K11, K12, K13
CTL Male B5, H5 H52, L1, L2 K14, K15, K16
TRT Female C4, C5 C42, E3, E7 K6, K8, K9
TRT Male C6, C7, C8, C9 K4, K17, K18 
coldata
##     treatment sex time     group
## B2        CTL   F  D10 CTL_F_D10
## B3        CTL   F  D10 CTL_F_D10
## B5        CTL   M  D10 CTL_M_D10
## C42       TRT   F  D10 TRT_F_D10
## C4        TRT   F  D10 TRT_F_D10
## C5        TRT   F  D10 TRT_F_D10
## C6        TRT   M  D10 TRT_M_D10
## C7        TRT   M  D10 TRT_M_D10
## C8        TRT   M  D10 TRT_M_D10
## C9        TRT   M  D10 TRT_M_D10
## E3        TRT   F  D10 TRT_F_D10
## E7        TRT   F  D10 TRT_F_D10
## H3        CTL   F  D10 CTL_F_D10
## H52       CTL   M  D10 CTL_M_D10
## H5        CTL   M  D10 CTL_M_D10
## H6        CTL   F  D10 CTL_F_D10
## K11       CTL   F   D3  CTL_F_D3
## K12       CTL   F   D3  CTL_F_D3
## K13       CTL   F   D3  CTL_F_D3
## K14       CTL   M   D3  CTL_M_D3
## K15       CTL   M   D3  CTL_M_D3
## K16       CTL   M   D3  CTL_M_D3
## K17       TRT   M   D3  TRT_M_D3
## K18       TRT   M   D3  TRT_M_D3
## K4        TRT   M   D3  TRT_M_D3
## K6        TRT   F   D3  TRT_F_D3
## K8        TRT   F   D3  TRT_F_D3
## K9        TRT   F   D3  TRT_F_D3
## L1        CTL   M  D10 CTL_M_D10
## L2        CTL   M  D10 CTL_M_D10

Alignment quality

Pre batch correction: heatmap and PCA

Post batch correction: heatmap and PCA

Comparisons

1. TRT_D3 vs CTL_D3

2. Female_D3 vs Male_D3

3. TRT_D10 vs CTL_D10

4. Female_D10 vs Male_D10

5. TRT_D3 vs TRT_D10

6. CTL_D3 vs CTL_D10

Differentially expressed genes

Up-regulated Down-regulated Total
TRT_D3 vs CTL_D3 3043 2202 5245
Female_D3 vs Male_D3 336 244 580
TRT_D10 vs CTL_D10 730 123 853
Female_D10 vs Male_D10 134 102 236
TRT_D3 vs TRT_D10 3076 2206 5282
CTL_D3 vs CTL_D10 593 334 927

Volcano plot

1. TRT_D3 vs CTL_D3, padj<0.05, FC>2

2. Female_D3 vs Male_D3, padj<0.05, FC>2

3. TRT_D10 vs CTL_D10, padj<0.05, FC>2

4. Female_D10 vs Male_D10, padj<0.05, FC>2

5. TRT_D3 vs TRT_D10, padj<0.05, FC>2

6. CTL_D3 vs CTL_D10, padj<0.05, FC>2

KEGG and GO