| General summary |
lib054_aln.sorted.bam |
lib055_aln.sorted.bam |
lib056_aln.sorted.bam |
lib057_aln.sorted.bam |
lib058_aln.sorted.bam |
lib059_aln.sorted.bam |
| Average percent identity |
88.7 |
89.2 |
88.8 |
89.4 |
89.7 |
89.5 |
| Fraction of bases aligned |
0.7 |
0.7 |
0.7 |
0.7 |
0.7 |
0.7 |
| Mean read length |
626.2 |
624.4 |
633.2 |
635.2 |
624.2 |
627.7 |
| Mean read quality |
9.4 |
9.8 |
9.4 |
9.8 |
10.1 |
9.9 |
| Median percent identity |
90.1 |
90.7 |
90.2 |
91.0 |
91.4 |
91.0 |
| Median read length |
541.0 |
537.0 |
542.0 |
514.0 |
503.0 |
515.0 |
| Median read quality |
10.1 |
10.5 |
10.0 |
10.6 |
10.9 |
10.7 |
| Number of reads |
37,232,035.0 |
39,014,323.0 |
47,443,672.0 |
56,587,154.0 |
57,687,882.0 |
40,929,869.0 |
| Read length N50 |
923.0 |
930.0 |
955.0 |
982.0 |
967.0 |
960.0 |
| STDEV read length |
557.2 |
565.3 |
573.5 |
590.0 |
582.9 |
578.7 |
| Total bases |
23,316,275,735.0 |
24,362,481,926.0 |
30,041,661,462.0 |
35,945,220,262.0 |
36,007,477,505.0 |
25,691,151,321.0 |
| Total bases aligned |
15,689,289,039.0 |
16,336,328,389.0 |
20,151,431,436.0 |
24,288,403,793.0 |
24,177,006,139.0 |
17,308,327,011.0 |
| Number, percentage and megabases of reads above quality cutoffs |
|
|
|
|
|
|
| >Q5 |
37221643 (100.0%) 23315.0Mb |
39006678 (100.0%) 24361.6Mb |
47431943 (100.0%) 30040.3Mb |
56573025 (100.0%) 35943.5Mb |
57675211 (100.0%) 36006.0Mb |
40919619 (100.0%) 25689.9Mb |
| >Q7 |
37047705 (99.5%) 23288.3Mb |
38879534 (99.7%) 24341.7Mb |
47195173 (99.5%) 30003.7Mb |
56372177 (99.6%) 35912.0Mb |
57510646 (99.7%) 35980.2Mb |
40789961 (99.7%) 25669.7Mb |
| >Q10 |
19427726 (52.2%) 11529.4Mb |
24014169 (61.6%) 14511.6Mb |
24220847 (51.1%) 14250.2Mb |
35029696 (61.9%) 21574.1Mb |
39843734 (69.1%) 24378.7Mb |
26300021 (64.3%) 16116.8Mb |
| >Q12 |
5431797 (14.6%) 1949.4Mb |
8191888 (21.0%) 3601.4Mb |
7318700 (15.4%) 2805.1Mb |
12673550 (22.4%) 5919.2Mb |
16386970 (28.4%) 8220.8Mb |
9621105 (23.5%) 4522.1Mb |
| >Q15 |
694393 (1.9%) 77.7Mb |
1178088 (3.0%) 138.7Mb |
983835 (2.1%) 112.1Mb |
1872736 (3.3%) 224.6Mb |
2607314 (4.5%) 325.0Mb |
1437608 (3.5%) 172.3Mb |
| Top 5 highest mean basecall quality scores and their read lengths |
|
|
|
|
|
|
| 1 |
29.4 (49) |
31.9 (44) |
31.5 (63) |
30.8 (49) |
30.8 (51) |
30.4 (61) |
| 2 |
28.8 (66) |
30.1 (71) |
29.5 (68) |
30.4 (52) |
30.7 (80) |
29.9 (50) |
| 3 |
28.7 (68) |
30.0 (64) |
28.4 (53) |
30.2 (46) |
30.6 (50) |
29.8 (50) |
| 4 |
28.6 (50) |
29.9 (44) |
28.2 (45) |
29.6 (49) |
30.5 (68) |
29.4 (45) |
| 5 |
28.6 (75) |
29.7 (64) |
28.1 (82) |
29.6 (65) |
30.5 (49) |
29.3 (61) |
| Top 5 longest reads and their mean basecall quality score |
|
|
|
|
|
|
| 1 |
10717 (9.1) |
261150 (7.8) |
30021 (7.4) |
69734 (7.6) |
95706 (7.4) |
42729 (7.3) |
| 2 |
10090 (8.8) |
30157 (7.4) |
26332 (7.4) |
51637 (7.6) |
29258 (7.9) |
16823 (7.8) |
| 3 |
9973 (10.2) |
24113 (7.7) |
16245 (8.5) |
13752 (7.6) |
20072 (8.1) |
15499 (7.9) |
| 4 |
9647 (9.1) |
15729 (7.3) |
14872 (8.6) |
13705 (7.8) |
16975 (10.9) |
14201 (7.9) |
| 5 |
9455 (8.3) |
13576 (7.8) |
14026 (7.3) |
13183 (7.4) |
12811 (11.2) |
13721 (7.4) |