FastQCFastQC Report
Tue 6 Aug 2024
trimmed_SRR1044415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_SRR1044415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3548528
Sequences flagged as poor quality0
Sequence length3-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGTAAAGAAGTATGTATTCGCG54172115.266076525252162No Hit
CATCGGTCGAACTTATGTGAAATCGCG840112.3674887164480594No Hit
TAGCATATCGAGCCTGAGAACATCGCG699101.970112677707489No Hit
AGGTTCCGGATAAGTAAGAGCCTCGCG681481.9204582858018875No Hit
TCTTAACCCGGACCAGAAACTATCGCG666131.8772009126037614No Hit
TGAGATGAAGCACTGTAGCTCTTCGCG637661.796970462118377No Hit
AAGCTGCCAGTTGAAGAACTGTTCGCG634311.7875299279024992No Hit
TGATACGGATGTTATACGCAGCTCGCG630671.7772721534112172No Hit
TTGGTCCCCTTCAACCAGCTGTTCGCG557701.571637591699995No Hit
TGAGGTAGTAGATTGTATAGTTTCGCG555671.5659169097721648No Hit
TTTGGTCCCCTTCAACCAGCTGTTCGCG532001.4992131948796796No Hit
TTAAGACTTGCAGTGATGTTTTCGCG518131.460126565156031No Hit
GATGTAACGAGTTGGAATGCAATCGCG462271.3027091796936645No Hit
TAGCTTATCAGACTGATGTTGACTCGCG443991.2511948616440394No Hit
TCGTACCGTGAGTAATAATGCGTCGCG416621.1740642880653611No Hit
TGAGGTAGTAGGTTGTATAGTTTCGCG383981.0820824860336455No Hit
GTCCCACTCCGTAGATCTGTTCTCGCG371241.0461802753141585No Hit
TGAGATGAAGCACTGTAGCTCTCGCG347450.9791383920318509No Hit
TAGCTTATCAGACTGATGTTGATCGCG334040.9413480744691883No Hit
ATAAGACGAACAAAAGGTTTGTTCGCG320800.9040368287921076No Hit
CATTATTACTTTTGGTACGCGTCGCG304370.8577359400855791No Hit
TTCAAGTAATCCAGGATAGGCTTCGCG284290.8011490961886167No Hit
TTCACAGTGGCTAAGTTCTGCTCGCG269400.7591880351514769No Hit
TGGAATGTAAAGAAGTATGTTCGCG235210.6628382247512208No Hit
TTAAGACTTGCAGTGATGTTTATCGCG234810.6617109967851458No Hit
TGGCTCAGTTCAGCAGGAACAGTTCGCG224330.6321776240739823No Hit
ACTGGACTTGGAGTCAGAAGGCTCGCG212190.5979662553036076No Hit
TAGCACCATTTGAAATCAGTGTTTCGCG197720.5571887836308463No Hit
TAGCACCATTTGAAATCGGTTTCGCG169150.47667652615394324No Hit
TTTGGTCCCCTTCAACCAGCTGTCGCG166430.4690113759846336No Hit
TAACTCCTTAAGCGAATCTCGCTCGCG165600.46667237795502814No Hit
TTCAAGTAATTCAGGATAGGTTTCGCG162200.45709094024339103No Hit
TAGCACCATCTGAAATCGGTTATCGCG161370.45475194221378556No Hit
TGGAATGTAAAGAAGTATGTATTTCGCG158500.4466640815571978No Hit
TGGAATGTAAAGAAGTATGTATCGCG157130.4428033257733911No Hit
TGAGGTAGTAGTTTGTACAGTTTCGCG156620.44136611011664556No Hit
TGTAAACATCCTTGACTGGAAGCTTCGCG155000.43680083685404203No Hit
CGTACCGTGAGTAATAATGCGTCGCG152840.43071380583723734No Hit
TGTAAACATCCTACACTCTCAGCTTCGCG142060.40033501215151746No Hit
TGAGATGAAGCACTGTAGCTTCGCG128850.36310830857189236No Hit
TGAGATGAAGCACTGTAGCTCATCGCG128010.36074112984313494No Hit
TGTAAACATCCTCGACTGGAAGCTTCGCG117190.3302496133608076No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCG115800.3263324961786972No Hit
TGGAATGTAAAGAAGTATGTAATCGCG112140.3160183602891114No Hit
AGCAGCATTGTACAGGGCTATGATCGCG110170.3104667625561923No Hit
TAGCACCATCTGAAATCGGTTTCGCG109950.3098467871748511No Hit
TGAGGTAGTAGATTGTATAGTTCGCG104540.29460102893368745No Hit
TCCCTGAGACCCTAACTTGTGATCGCG98610.2778898743366263No Hit
TGAGGTAGTAGGTTGTATAGTTCGCG98460.2774671638493482No Hit
TGTAAACATCCTACACTCAGCTTCGCG94920.26749119634958496No Hit
AAAGTAGCATCCGAAATACGGATCGCG93950.2647576685318532No Hit
TAGCACCATTTGAAATCAGTGTTCGCG93520.2635458984683226No Hit
TGTAAACATCCCCGACTGGAAGCTTCGCG91800.2586988182142004No Hit
TGTAAACATCCTTGACTGGAAGCTCGCG82450.23234986450719847No Hit
TGTAAACATCCTACACTCTCAGCTCGCG82140.23147626283349038No Hit
AACCCGTAGATCCGATCTTGTTCGCG79360.2236420284692695No Hit
TCAGTGCACTACAGAACTTTGTTCGCG75270.21211612251615317No Hit
ACTGGACTTGGAGTCAGAAGGTCGCG73750.2078326562450684No Hit
TGTGCAAATCCATGCAAAACTGATCGCG71440.20132291474098557No Hit
TGTAAACATCCTCGACTGGAAGCTCGCG67180.18931793690228738No Hit
TGTAAACATCCCCGACTGGAATCGCG66750.1881061668387568No Hit
GGAATGTAAAGAAGTATGTATTCGCG65850.18556990391508818No Hit
ATAAGACGAGCAAAAAGCTTGTTCGCG65760.18531627762272132No Hit
TACAGTACTGTGATAACTGAAGTCGCG62360.17573483991108427No Hit
TGTAAACATCCCCGACTGGAAGCTCGCG62270.17548121361871738No Hit
TTCACAGTGGCTAAGTTCCGTCGCG59320.16716790736891465No Hit
TTCACAGTGGCTAAGTTCCGCTCGCG58820.16575887241132098No Hit
TGTAAACATCCCCGACTGGAAGTCGCG56620.15955911859790878No Hit
ACAGTAGTCTGCACATTGGTTTCGCG55680.15691013287763264No Hit
TGAGGTAGTAGTTTGTGCTGTTTCGCG55410.15614925400053203No Hit
TGAGATGAAGCACTGTAGCTCTTTCGCG55260.15572654351325396No Hit
AGAGGTAGTAGGTTGCATAGTTTCGCG55020.15505020673360898No Hit
TCGTACCGTGAGTAATAATGCGATCGCG53570.1509640053565873No Hit
TGGCTCAGTTCAGCAGGAACTCGCG53330.1502876685769423No Hit
TTCAAGTAATCCAGGATAGGCTTTCGCG52660.14839956173376678No Hit
TTTGGTCCCCTTCAACCAGCTTCGCG51550.1452715041279088No Hit
TTGGTCCCCTTCAACCAGCTGTTTCGCG50790.1431297709923664No Hit
TGTAAACATCCTCGACTGGAAGTCGCG50260.1416361939373171No Hit
ATCACATTGCCAGGGATTACCATCGCG48330.13619731900100548No Hit
TTTGGTCCCCTTCAACCAGCTGATCGCG47550.13399922446715934No Hit
TAGCAGCACGTAAATATTGGCGTCGCG47380.13352015258157748No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATCGCG46820.13194203342907257No Hit
TTCACAGTGGCTAAGTTCTGCATCGCG45180.12732039876816528No Hit
TTGGTCCCCTTCAACCAGCTGTATCGCG44410.125150484933471No Hit
TGTAAACATCCTCGACTGGAATCGCG42330.11928889950988128No Hit
TAGCTTATCAGACTGATGTTGTCGCG41770.11771078035737635No Hit
CATTATTACTTTTGGTACGCGATCGCG41190.11607629980656768No Hit
TAGCACCATTTGAAATCGGTTATCGCG40680.11463908414982213No Hit
ATAAGACGAACAAAAGGTTTGTCGCG39630.11168011073887539No Hit
CGTACCGTGAGTAATAATGCGATCGCG38720.10911566711605489No Hit
AAACCGTTACCATTACTGAGTTCGCG37520.10573398321783005No Hit
ATCACATTGCCAGGGATTACCACTTCGCG37100.10455039385345134No Hit
CAGTGCAATGTTAAAAGGGCATTCGCG36820.10376133427719889No Hit
TGAGGTAGTAGGTTGTATGGTTTCGCG36330.10238048001875709No Hit
TGAGGTAGTAGGTTGTGTGGTTTCGCG36110.10176050463741586No Hit
TTTGGTCCCCTTCAACCAGCTGTTTCGCG35750.1007459994679484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)