FastQCFastQC Report
Tue 6 Aug 2024
trimmed_SRR1044416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_SRR1044416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5826954
Sequences flagged as poor quality0
Sequence length3-51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGTAAAGAAGTATGTATTCTAG81323413.956417023371046No Hit
AAGCTGCCAGTTGAAGAACTGTTCTAG1338752.2975125597353268No Hit
TGAGATGAAGCACTGTAGCTCTTCTAG1208122.073330251105466No Hit
TGAGGTAGTAGATTGTATAGTTTCTAG1049471.8010610689564395No Hit
TTAAGACTTGCAGTGATGTTTTCTAG1023931.7572302784610965No Hit
CATCGGTCGAACTTATGTGAAATCTAG1008831.7313162245660425No Hit
TGAGGTAGTAGGTTGTATAGTTTCTAG847061.4536926153870444No Hit
TGATACGGATGTTATACGCAGCTCTAG821031.4090209052619946No Hit
TTGGTCCCCTTCAACCAGCTGTTCTAG816281.400869133341365No Hit
TAGCATATCGAGCCTGAGAACATCTAG804991.3814936586079107No Hit
TTCACAGTGGCTAAGTTCTGCTCTAG753421.292991157987518No Hit
AGGTTCCGGATAAGTAAGAGCCTCTAG740371.270595237237157No Hit
TAGCTTATCAGACTGATGTTGACTCTAG711011.220208705955118No Hit
TGAGATGAAGCACTGTAGCTCTCTAG695611.1937798033071825No Hit
TTTGGTCCCCTTCAACCAGCTGTTCTAG666171.1432559790243755No Hit
TTCAAGTAATCCAGGATAGGCTTCTAG615831.0568643582907984No Hit
TAGCTTATCAGACTGATGTTGATCTAG550360.9445071987868792No Hit
TGGCTCAGTTCAGCAGGAACAGTTCTAG512380.8793273466720349No Hit
ATAAGACGAACAAAAGGTTTGTTCTAG497690.8541169194059195No Hit
GATGTAACGAGTTGGAATGCAATCTAG497110.8531215451503479No Hit
TCTTAACCCGGACCAGAAACTATCTAG483720.8301421291467206No Hit
TGGAATGTAAAGAAGTATGTTCTAG465830.7994399818498653No Hit
TCCCTGAGACCCTAACTTGTGATCTAG454300.7796526281141055No Hit
GTCCCACTCCGTAGATCTGTTCTCTAG434570.7457927417995749No Hit
TTAAGACTTGCAGTGATGTTTATCTAG425430.730107016461774No Hit
TAGCACCATTTGAAATCGGTTTCTAG404280.6938101793836025No Hit
TCGTACCGTGAGTAATAATGCGTCTAG379960.6520731071499792No Hit
ACTGGACTTGGAGTCAGAAGGCTCTAG350050.6007426864876572No Hit
TGTAAACATCCTACACTCTCAGCTTCTAG339020.5818134140067006No Hit
TGTAAACATCCTTGACTGGAAGCTTCTAG335220.575291996470197No Hit
TAGCACCATCTGAAATCGGTTATCTAG298850.5128751659958187No Hit
TGAGGTAGTAGTTTGTACAGTTTCTAG277070.47549714653659525No Hit
TGGAATGTAAAGAAGTATGTATCTAG274590.4712410635127719No Hit
TTCAAGTAATTCAGGATAGGTTTCTAG271370.46571502023183986No Hit
TGGCTCAGTTCAGCAGGAACAGTCTAG256610.440384461590052No Hit
TAGCACCATTTGAAATCAGTGTTTCTAG253240.43460099393267904No Hit
TTCAAGTAATCCAGGATAGGCTCTAG252720.43370858942768387No Hit
TGAGATGAAGCACTGTAGCTTCTAG244150.4190010767203585No Hit
TGGAATGTAAAGAAGTATGTATTTCTAG240130.41210210343174153No Hit
TGTAAACATCCTCGACTGGAAGCTTCTAG236930.40661038340100164No Hit
TGTAAACATCCTACACTCAGCTTCTAG231630.3975147221000887No Hit
TGAGGTAGTAGGTTGTATAGTTCTAG225480.3869603226660104No Hit
TGAGATGAAGCACTGTAGCTCATCTAG225070.38625669603707186No Hit
TGTAAACATCCTACACTCTCAGCTCTAG224850.38587914028495846No Hit
TGAGGTAGTAGATTGTATAGTTCTAG221510.3801471575028737No Hit
TAGCACCATCTGAAATCGGTTTCTAG212420.3645472402905532No Hit
TGTAAACATCCCCGACTGGAAGCTTCTAG205380.3524654562229254No Hit
TGGCTCAGTTCAGCAGGAACTCTAG203760.34968527295736335No Hit
AAAGTAGCATCCGAAATACGGATCTAG201580.34594403868642176No Hit
TGTAAACATCCTTGACTGGAAGCTCTAG201290.34544635155863596No Hit
CATTATTACTTTTGGTACGCGTCTAG200700.34443381567796827No Hit
TGGAATGTAAAGAAGTATGTAATCTAG186800.3205791567944418No Hit
TAACTCCTTAAGCGAATCTCGCTCTAG183210.3144181333849555No Hit
TGTAAACATCCCCGACTGGAAGCTCTAG174330.29917861029965226No Hit
ACTGGACTTGGAGTCAGAAGGTCTAG171400.2941502541465061No Hit
AACCCGTAGATCCGATCTTGTTCTAG169800.2914043941311361No Hit
TAGCACCATTTGAAATCAGTGTTCTAG165440.283921925589253No Hit
TGTAAACATCCTCGACTGGAAGCTCTAG163230.28012920644302325No Hit
TTCACAGTGGCTAAGTTCCGCTCTAG157360.2700553325116347No Hit
ATCACATTGCCAGGGATTACCACTCTAG140270.24072611522246443No Hit
AGCAGCATTGTACAGGGCTATGATCTAG133200.22859284627954846No Hit
TCGTACCGTGAGTAATAATGCGATCTAG128080.2198060942303646No Hit
TAGCACCATTTGAAATCGGTTATCTAG122780.21071043292945163No Hit
TCAGTGCACTACAGAACTTTGTTCTAG119450.20499561177246295No Hit
TACAGTACTGTGATAACTGAAGTCTAG119060.2043263083937165No Hit
ATCACATTGCCAGGGATTACCTCTAG118990.20420617701804405No Hit
TGAGATGAAGCACTGTAGCTCTTTCTAG118040.20257582263391816No Hit
ATCACATTGCCAGGGATTACCACTTCTAG111490.19133495819599744No Hit
TTTGGTCCCCTTCAACCAGCTTCTAG111070.1906141699419628No Hit
TGAGGTAGTAGGTTGTGTGGTTTCTAG110570.1897560886871597No Hit
ATAAGACGAGCAAAAAGCTTGTTCTAG107890.18515677316141504No Hit
TGAGGTAGTAGTTTGTGCTGTTTCTAG105910.1817587713923947No Hit
AGAGGTAGTAGGTTGCATAGTTTCTAG104360.17909871950250508No Hit
ATAAGACGAACAAAAGGTTTGTCTAG103180.17707364774116974No Hit
CATTATTACTTTTGGTACGCGATCTAG103100.17693635474040126No Hit
TTCACAGTGGCTAAGTTCTGCATCTAG101330.17389874709839823No Hit
ATCACATTGCCAGGGATTTCCTCTAG99300.1704149372038976No Hit
TGAGGTAGTAGGTTGTATGGTTTCTAG96030.1648030857974853No Hit
GGAATGTAAAGAAGTATGTATTCTAG94880.16282949891143814No Hit
ACAGTAGTCTGCACATTGGTTTCTAG94350.1619199327813468No Hit
CGTACCGTGAGTAATAATGCGTCTAG92920.15946582039260992No Hit
TTCACAGTGGCTAAGTTCTGCTTCTAG91820.1575780416320431No Hit
TTTGGTCCCCTTCAACCAGCTGTCTAG91220.15654834412627935No Hit
TTCAAGTAATCCAGGATAGGCTTTCTAG87830.15073055321871426No Hit
TAGCAGCACGTAAATATTGGCGTCTAG86950.1492203302102608No Hit
TGTGCAAATCCATGCAAAACTGATCTAG84290.14465533793470825No Hit
ACTGGACTTGGAGTCAGAAGTCTAG84000.14415765080692244No Hit
TGTAAACATCCCCGACTGGAATCTAG81680.14017615378463602No Hit
AGCTGGTAAAATGGAACCAAATTCTAG81090.13916361790396836No Hit
GTACAGTACTGTGATAACTGATCTAG80820.13870025402637468No Hit
ATCACATTGCCAGGGATTACCATCTAG80720.13852863777541405No Hit
ACAGTAGTCTGCACATTGGTTATCTAG77490.13298543286938597No Hit
TTTGGTCCCCTTCAACCAGCTGATCTAG77310.13267652361765686No Hit
TTCACAGTGGCTAAGTTCTGTTCTAG76880.1319385737385262No Hit
TCCCTGAGACCCTTTAACCTGTTCTAG76740.1316983109871813No Hit
TAGCTTATCAGACTGATGTTGTCTAG75820.1301194414783436No Hit
TGTAAACATCCCCGACTGGATCTAG75040.12878083472085072No Hit
TGGCTCAGTTCAGCAGGAACAGATCTAG74880.1285062487193137No Hit
TTGGTCCCCTTCAACCAGCTGTTTCTAG74200.1273392582127815No Hit
TTCACAGTGGCTAAGTTCTGTCTAG73360.12589768170471227No Hit
CCCAGTGTTCAGACTACCTGTTCTCTAG73110.12546864107731073No Hit
TGTAAACATCCCCGACTGGAAGTCTAG72940.12517689345067765No Hit
AACCCGTAGATCCGATCTTGTGTCTAG72410.12426732732058636No Hit
TTGGTCCCCTTCAACCAGCTGTATCTAG69050.11850102128830946No Hit
CAGTGCAATGTTAAAAGGGCATTCTAG68380.11735119240687329No Hit
CGTACCGTGAGTAATAATGCGATCTAG68050.11678485877870326No Hit
GTCCAGTTTTCCCAGGAATCCCTTCTAG67720.1162185251505332No Hit
TTCACAGTGGCTAAGTTCCGTCTAG65430.11228851300353496No Hit
AAACCGTTACCATTACTGAGTTTCTAG64340.11041789586806418No Hit
AAACCGTTACCATTACTGAGTTCTAG63250.10854727873259339No Hit
AGCTACATTGTCTGCTGGGTTTTCTAG63120.1083241776063446No Hit
TGAGGTAGGAGGTTGTATAGTTTCTAG62730.10765487422759816No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATCTAG61440.10544102459020613No Hit
TTCAAGTAATTCAGGATAGGTTCTAG60880.10447997358482665No Hit
ATCACATTGCCAGGGATTTCCATCTAG60050.1030555587018535No Hit
TGGAATGTAAAGAAGTATGTACTCTAG58970.10120210319147878No Hit
TGTAAACATCCTCGACTGGAAGTCTAG58690.10072157768878903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)