FastQCFastQC Report
Tue 6 Aug 2024
trimmed_SRR1044417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_SRR1044417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7484914
Sequences flagged as poor quality0
Sequence length4-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGTAAAGAAGTATGTATTCACT111924614.953358181536888No Hit
AAGCTGCCAGTTGAAGAACTGTTCACT1793222.395779029658858No Hit
TGATACGGATGTTATACGCAGCTCACT1644322.1968455482588043No Hit
TGAGATGAAGCACTGTAGCTCTTCACT1446801.9329547406957515No Hit
TTGGTCCCCTTCAACCAGCTGTTCACT1285111.7169335546139874No Hit
AGGTTCCGGATAAGTAAGAGCCTCACT1263141.687581179957445No Hit
TTCACAGTGGCTAAGTTCTGCTCACT1223781.6349954054248317No Hit
TAGCATATCGAGCCTGAGAACATCACT1126471.5049872316502233No Hit
TGAGATGAAGCACTGTAGCTCTCACT1046341.397931893405856No Hit
CATCGGTCGAACTTATGTGAAATCACT1035751.383783434251883No Hit
TTCAAGTAATCCAGGATAGGCTTCACT982921.3132014609653497No Hit
TGGAATGTAAAGAAGTATGTTCACT921221.2307689841192564No Hit
TTAAGACTTGCAGTGATGTTTTCACT888601.1871879890670753No Hit
ACTGGACTTGGAGTCAGAAGGCTCACT857431.1455442240218126No Hit
TTTGGTCCCCTTCAACCAGCTGTTCACT847001.1316095281789478No Hit
TCGTACCGTGAGTAATAATGCGTCACT837131.1184230039249616No Hit
TGAGGTAGTAGATTGTATAGTTTCACT764481.021361100474902No Hit
TGGCTCAGTTCAGCAGGAACAGTTCACT741200.9902585387086611No Hit
TAGCTTATCAGACTGATGTTGATCACT576990.7708705804769433No Hit
TGTAAACATCCTTGACTGGAAGCTTCACT558640.7463546007342235No Hit
GTCCCACTCCGTAGATCTGTTCTCACT542800.7251920329345133No Hit
ATAAGACGAACAAAAGGTTTGTTCACT518780.6931008158543973No Hit
TAGCTTATCAGACTGATGTTGACTCACT491240.6563068059299011No Hit
TGAGATGAAGCACTGTAGCTTCACT477490.6379365213815416No Hit
TGAGGTAGTAGGTTGTATAGTTTCACT441370.5896794538988691No Hit
TGGAATGTAAAGAAGTATGTATCACT426560.5698929874144179No Hit
TTAAGACTTGCAGTGATGTTTATCACT405290.5414758272439737No Hit
GATGTAACGAGTTGGAATGCAATCACT383660.512577699623536No Hit
TAGCACCATTTGAAATCGGTTTCACT370390.49484870500850114No Hit
TAGCACCATCTGAAATCGGTTATCACT361960.4835860505544886No Hit
TGTAAACATCCTTGACTGGAAGCTCACT357530.4776674788781808No Hit
TGAGATGAAGCACTGTAGCTCATCACT354320.47337885244907285No Hit
AAAGTAGCATCCGAAATACGGATCACT333840.4460171486272254No Hit
TCCCTGAGACCCTAACTTGTGATCACT333380.44540257910778935No Hit
TGTAAACATCCCCGACTGGAAGCTCACT324170.4330978285121245No Hit
TTCAAGTAATTCAGGATAGGTTTCACT320310.4279407886316396No Hit
TGGCTCAGTTCAGCAGGAACAGTCACT319740.4271792568358167No Hit
CATTATTACTTTTGGTACGCGTCACT315290.4212339647456203No Hit
ACTGGACTTGGAGTCAGAAGGTCACT312850.41797407425122046No Hit
TGGAATGTAAAGAAGTATGTATTTCACT310740.4151550705859814No Hit
TAGCACCATCTGAAATCGGTTTCACT308580.41226926588602086No Hit
TGTAAACATCCTCGACTGGAAGCTTCACT300150.40100661143200844No Hit
TGGAATGTAAAGAAGTATGTAATCACT295120.3942864273390449No Hit
TCGTACCGTGAGTAATAATGCGATCACT290970.3887419414571764No Hit
TGTAAACATCCTCGACTGGAAGCTCACT269170.35961669031868637No Hit
CGTACCGTGAGTAATAATGCGTCACT266680.35628999878956524No Hit
AGCAGCATTGTACAGGGCTATGATCACT261800.34977021780076567No Hit
TGTAAACATCCCCGACTGGAAGCTTCACT259780.3470714559980248No Hit
TGGCTCAGTTCAGCAGGAACTCACT248340.33178737925378965No Hit
TTCAAGTAATCCAGGATAGGCTCACT246530.32936918179687835No Hit
TTCACAGTGGCTAAGTTCCGTCACT220100.29405815484319525No Hit
TTCACAGTGGCTAAGTTCCGCTCACT217620.29074482352101844No Hit
TCAGTGCACTACAGAACTTTGTTCACT216930.28982296924186435No Hit
TGAGGTAGTAGATTGTATAGTTCACT215970.28854038937521526No Hit
TAACTCCTTAAGCGAATCTCGCTCACT213510.2852537784669269No Hit
TAGCAGCACGTAAATATTGGCGTCACT210880.28174004404058617No Hit
GTACAGTACTGTGATAACTGATCACT207810.2776384605086979No Hit
TGAGGTAGTAGTTTGTACAGTTTCACT194330.2596289015478334No Hit
AAACCGTTACCATTACTGAGTTTCACT185150.24736423157300136No Hit
TCTTAACCCGGACCAGAAACTATCACT173360.2316125475857171No Hit
TGTAAACATCCTACACTCTCAGCTCACT161520.21579406256371148No Hit
TTCACAGTGGCTAAGTTCTGCTTCACT160090.2138835529706821No Hit
AACCCGTAGATCCGATCTTGTTCACT155960.2083657875027021No Hit
TGAGGTAGTAGGTTGTATAGTTCACT153780.20545326238885311No Hit
TTCACAGTGGCTAAGTTCTGCATCACT153560.20515933783607937No Hit
CGTACCGTGAGTAATAATGCGATCACT152190.20332898948471553No Hit
TACAGTACTGTGATAACTGAAGTCACT144820.1934825169667948No Hit
TTCACAGTGGCTAAGTTCTGTCACT137080.18314171679193642No Hit
TTTGGTCCCCTTCAACCAGCTGTCACT135090.1804830356100284No Hit
AAACCGTTACCATTACTGAGTTCACT130750.1746847057962189No Hit
TGTAAACATCCCCGACTGGAAGTCACT129180.17258715330596985No Hit
TTCAAGTAATCCAGGATAGGCTTTCACT128780.1720527450281994No Hit
TGTAAACATCCTACACTCTCAGCTTCACT127310.17008879460739296No Hit
GTACAGTACTGTGATAACTGAATCACT125890.16819164522130783No Hit
TAGCACCATTTGAAATCAGTGTTTCACT124790.16672202245743906No Hit
ACAGTAGTCTGCACATTGGTTTCACT120600.1611240957477935No Hit
TTTGGTCCCCTTCAACCAGCTTCACT118680.15855893601449528No Hit
ACTGGACTTGGAGTCAGAAGGATCACT118170.15787756546033793No Hit
ACTGGACTTGGAGTCAGAAGTCACT117170.1565415447659118No Hit
TGAGGTAGTAGTTTGTGCTGTTTCACT112410.15018208626044335No Hit
GTCCAGTTTTCCCAGGAATCCCTTCACT111430.14887278597990572No Hit
TGGCTCAGTTCAGCAGGAACAGATCACT111240.14861894204796475No Hit
TACAGTACTGTGATAACTGATCACT110320.14738980300909268No Hit
TTGGTCCCCTTCAACCAGCTGTTTCACT106920.1428473326480438No Hit
TGAGATGAAGCACTGTAGCTCTTTCACT103780.13865222766754567No Hit
TTCACAGTGGCTAAGTTCTGTTCACT102180.13651459455646384No Hit
TGTAAACATCCTTGACTGGATCACT99150.13246645185235262No Hit
TCGTACCGTGAGTAATAATGTCACT97080.12970088901489046No Hit
TGGAATGTAAAGAAGTATGTACTCACT96940.12951384611767083No Hit
TGTGCAAATCCATGCAAAACTGTCACT96760.12927336239267412No Hit
ATCACATTGCCAGGGATTACCTCACT96730.12923328177184135No Hit
CAGCAGCACACTGTGGTTTGTTCACT96630.12909967970239872No Hit
ATAAGACGAGCAAAAAGCTTGTTCACT92620.12374223671774986No Hit
TTCAAGTAATTCAGGATAGGTTCACT92040.1229673447149827No Hit
TTCAAGTAATCCAGGATAGGTCACT90960.12152444236500247No Hit
TTGAATGTAAAGAAGTATGTATTCACT89550.11964065318586159No Hit
TACAGTACTGTGATAACTGAATCACT89540.11962729297891733No Hit
ACTGGACTTGGAGTCAGAAGGCATCACT88470.11819775083588134No Hit
TGTAAACATCCTACACTCAGCTTCACT86680.11580627379285854No Hit
TGTAAACATCCCCGACTGGATCACT86480.11553906965397331No Hit
CATTATTACTTTTGGTACGCGATCACT85090.11368200088872098No Hit
TGTAAACATCCTCGACTGGAAGTCACT83350.11135732488041948No Hit
TGTAAACATCCTCGACTGGATCACT83250.11122372281097685No Hit
ATCACATTGCCAGGGATTTCCTCACT83140.11107676053458998No Hit
TGGGATGTAAAGAAGTATGTATTCACT79990.1068682953471476No Hit
CAGTGCAATGTTAAAAGGGCATTCACT79560.10629380644854437No Hit
TAGCTTATCAGACTGATGTTGTCACT78840.10533187154855754No Hit
CTTTCAGTCGGATGTTTACAGTTCACT77750.10387560899163303No Hit
TACAGTACTGTGATAACTGAATTCACT76970.10283351284998066No Hit
TTGGTCCCCTTCAACCAGCTGTATCACT76540.1022590239513774No Hit
GGAATGTAAAGAAGTATGTATTCACT76280.1019116585708266No Hit
TATTGCACTTGTCCCGGCCTGTTCACT75840.1013238094652791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)