FastQCFastQC Report
Tue 6 Aug 2024
trimmed_SRR1044418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_SRR1044418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8581965
Sequences flagged as poor quality0
Sequence length0-51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGTAAAGAAGTATGTATTTGGT8273829.640938875886816No Hit
AAGCTGCCAGTTGAAGAACTGTTTGGT2767743.2250655881257964No Hit
TTGGTCCCCTTCAACCAGCTGTTTGGT1544041.799168372278377No Hit
CATCGGTCGAACTTATGTGAAATTGGT1528861.781480115567938No Hit
TTCAAGTAATCCAGGATAGGCTTTGGT1480761.725432345622477No Hit
TTAAGACTTGCAGTGATGTTTTTGGT1436521.6738823800842815No Hit
TGATACGGATGTTATACGCAGCTTGGT1267371.476782997833247No Hit
TTTGGTCCCCTTCAACCAGCTGTTTGGT1214041.4146410524862314No Hit
TGAGATGAAGCACTGTAGCTCTTTGGT1195961.3935736162988313No Hit
TGAGGTAGTAGGTTGTATAGTTTTGGT1159261.350809517400735No Hit
TGAGGTAGTAGATTGTATAGTTTTGGT1147461.33705975263241No Hit
TAGCATATCGAGCCTGAGAACATTGGT995231.1596761347779907No Hit
TCTTAACCCGGACCAGAAACTATTGGT980541.1425588428757283No Hit
TTCACAGTGGCTAAGTTCTGCTTGGT965801.125383289258346No Hit
AGGTTCCGGATAAGTAAGAGCCTTGGT906971.056832555248128No Hit
GATGTAACGAGTTGGAATGCAATTGGT804140.9370115119322906No Hit
TCGTACCGTGAGTAATAATGCGTTGGT795800.927293457850271No Hit
TGGCTCAGTTCAGCAGGAACAGTTTGGT705380.8219329722272231No Hit
TAGCACCATTTGAAATCGGTTTTGGT688370.8021123367433914No Hit
TAGCACCATTTGAAATCGGTTATTGGT661390.7706743152646276No Hit
AAAGTAGCATCCGAAATACGGATTGGT660560.7697071707936353No Hit
TGAGATGAAGCACTGTAGCTCTTGGT612420.713612791476078No Hit
ACTGGACTTGGAGTCAGAAGGCTTGGT607030.7073321785861396No Hit
ATAAGACGAACAAAAGGTTTGTTTGGT591920.689725488276869No Hit
TTAAGACTTGCAGTGATGTTTATTGGT547010.6373948157560652No Hit
TAGCTTATCAGACTGATGTTGATTGGT527840.6150572741790488No Hit
TGGAATGTAAAGAAGTATGTTTGGT522300.6086018761437503No Hit
GTCCCACTCCGTAGATCTGTTCTTGGT519790.6056771380447251No Hit
TGTAAACATCCTTGACTGGAAGCTTTGGT467080.5442576379651979No Hit
TCCCTGAGACCCTAACTTGTGATTGGT433830.5055135974103833No Hit
TGGCTCAGTTCAGCAGGAACAGTTGGT408630.4761496929898921No Hit
TAGCTTATCAGACTGATGTTGACTTGGT406290.4734230447222752No Hit
CATTATTACTTTTGGTACGCGTTGGT372530.43408473467323627No Hit
TTCAAGTAATTCAGGATAGGTTTTGGT363280.4233063173760322No Hit
TGAGATGAAGCACTGTAGCTTTGGT362050.4218730791840796No Hit
CGTACCGTGAGTAATAATGCGTTGGT344280.4011668656304238No Hit
TGGAATGTAAAGAAGTATGTATTGGT340240.396459319048726No Hit
TAGCAGCACGTAAATATTGGCGTTGGT316390.3686684809364755No Hit
TGAGGTAGTAGTTTGTACAGTTTTGGT294640.3433246348592659No Hit
TGAGATGAAGCACTGTAGCTCATTGGT280980.32740753428847585No Hit
TAGCACCATCTGAAATCGGTTTTGGT278890.324972194596459No Hit
TGAGGTAGTAGGTTGTATAGTTTGGT278500.3245177532185228No Hit
TGAGGTAGTAGATTGTATAGTTTGGT269690.31425203901437493No Hit
TGGAATGTAAAGAAGTATGTATTTTGGT267180.3113273009153498No Hit
TGTAAACATCCTTGACTGGAAGCTTGGT260330.3033454459439068No Hit
AACCCGTAGATCCGATCTTGTTTGGT256770.2991972118273612No Hit
TGTAAACATCCTCGACTGGAAGCTTTGGT247740.28867514607668526No Hit
TAGCACCATCTGAAATCGGTTATTGGT244660.28508622442529186No Hit
TAGCACCATTTGAAATCAGTGTTTTGGT235420.2743194594711118No Hit
TGGAATGTAAAGAAGTATGTAATTGGT224590.26169997197611505No Hit
TAACTCCTTAAGCGAATCTCGCTTGGT224420.26150188214470693No Hit
AACCCGTAGATCCGATCTTGTGTTGGT222150.25885680027825797No Hit
ACTGGACTTGGAGTCAGAAGGTTGGT221920.25858879638870585No Hit
TTTGGTCCCCTTCAACCAGCTGTTGGT221280.25784304643516953No Hit
TGTAAACATCCTACACTCTCAGCTTGGT221220.25777313237702554No Hit
TCAGTGCACTACAGAACTTTGTTTGGT211530.24648201198676525No Hit
TGTAAACATCCCCGACTGGAAGCTTGGT193670.2256709273458934No Hit
TGTAAACATCCCCGACTGGAAGCTTTGGT189530.2208468573339556No Hit
TGTAAACATCCTCGACTGGAAGCTTGGT189390.2206837245316195No Hit
CATTATTACTTTTGGTACGCGATTGGT186810.21767742003142637No Hit
ATAAGACGAGCAAAAAGCTTGTTTGGT186760.21761915831630635No Hit
TTCACAGTGGCTAAGTTCCGCTTGGT185500.2161509630952818No Hit
AGCAGCATTGTACAGGGCTATGATTGGT172810.2013641397978202No Hit
AGAGGTAGTAGGTTGCATAGTTTTGGT169040.19697120647777056No Hit
TTCAAGTAATCCAGGATAGGCTTGGT168300.19610893309399421No Hit
TACAGTACTGTGATAACTGAAGTTGGT167940.19568944874513006No Hit
TGAGGTAGTAGGTTGTGTGGTTTTGGT162820.18972344911683978No Hit
TGTAAACATCCCCGACTGGAATTGGT155190.18083271138952445No Hit
TGTAAACATCCTACACTCTCAGCTTTGGT152310.17747683659861116No Hit
ATCACATTGCCAGGGATTACCACTTTGGT143550.1672693841095833No Hit
ATCACATTGCCAGGGATTTCCTTGGT141270.1646126499001103No Hit
TGGCTCAGTTCAGCAGGAACTTGGT135210.1575513300275636No Hit
ATCACATTGCCAGGGATTACCTTGGT134800.15707358396357946No Hit
TTCACAGTGGCTAAGTTCTGCATTGGT129360.15073470935852104No Hit
TAGCACCATTTGAAATCAGTGTTTGGT129190.15053661952711297No Hit
ATCACATTGCCAGGGATTTCCATTGGT126950.14792649468973595No Hit
TGTAAACATCCCCGACTGGAAGTTGGT126670.14760022908506387No Hit
TGAGGTAGTAGGTTGTATGGTTTTGGT125780.14656317055592746No Hit
TTCACAGTGGCTAAGTTCTGCTTTGGT123310.14368504182899838No Hit
TTCAAGTAATCCAGGATAGGCTTTTGGT122760.14304416296267813No Hit
GTACAGTACTGTGATAACTGATTGGT121990.1421469325498298No Hit
TCGTACCGTGAGTAATAATGCGATTGGT121120.1411331787067414No Hit
TGTAAACATCCTCGACTGGAAGTTGGT115820.13495743690401907No Hit
GTCCAGTTTTCCCAGGAATCCCTTTGGT113790.13259201127014617No Hit
TCGTACCGTGAGTAATAATGTTGGT112800.13143842931076974No Hit
TTGGTCCCCTTCAACCAGCTGTTTTGGT111260.12964396848507306No Hit
TCGTACCGTGAGTAATAATGCTTGGT108530.12646287883951987No Hit
TTTGGTCCCCTTCAACCAGCTTTGGT108500.12642792181044785No Hit
TGTAAACATCCTTGACTGGAAGTTGGT107630.12541416796735946No Hit
TGTAAACATCCCCGACTGGATTGGT106650.12427223835100702No Hit
ATAAGACGAACAAAAGGTTTGTTGGT104950.12229134003692627No Hit
TGGAATGTAAAGAAGTATGTGTTTGGT103910.12107949636242983No Hit
ACTGGACTTGGAGTCAGAAGTTGGT100880.11754883642615649No Hit
TGTGCAAATCCATGCAAAACTGATTGGT95470.1112449188501701No Hit
GGAATGTAAAGAAGTATGTATTTGGT95290.111035176675738No Hit
ATCACATTGCCAGGGATTACCATTGGT94960.11065064935594586No Hit
TGTAAACATCCTCGACTGGAATTGGT93080.10846000886743305No Hit
ATCACATTGCCAGGGATTACCACTTGGT91690.10684033318709642No Hit
TTCAAGTAATCCAGGATAGGTTGGT91080.10612954026263217No Hit
TGTAAACATCCTACACTCAGCTTTGGT89870.10471960675672762No Hit
AACCCGTAGATCCGATCTTGTGATTGGT89500.10428847006483946No Hit
TTCAAGTAATTCAGGATAGGTTTGGT88480.10309993107639101No Hit
TGAGGTAGTAGTTTGTGCTGTTTTGGT87900.10242409518099876No Hit
CAGCAGCACACTGTGGTTTGTTTGGT87880.10240079049495074No Hit
TGGCTCAGTTCAGCAGGAACAGATTGGT87460.10191139208794256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)