FastQCFastQC Report
Tue 6 Aug 2024
trimmed_SRR1044427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_SRR1044427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3282957
Sequences flagged as poor quality0
Sequence length0-51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCATC75248822.921043437364546No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTCATC47807214.562237641248425No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAAAACTTCATC1520984.632957422226365No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTCATC900972.74438562551992No Hit
TAGCTTATCAGACTGATGTTGATCATC750022.284586730803967No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTCATC583931.7786708750678122No Hit
TATTGCACTTGTCCCGGCCTGTTCATC547731.6684044292995612No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAAAACTCATC402051.2246581359426882No Hit
TTCAAGTAATCCAGGATAGGCTTCATC338511.0311131093096864No Hit
TAGCATATCGAGCCTGAGAACATCATC304330.9269996530566803No Hit
CATTATTACTTTTGGTACGCGTCATC276290.8415888481024881No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGACCTCATC251150.7650115429474099No Hit
TGAGGTAGTAGGTTGTATAGTTTCATC236530.7204785198222212No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATCATC236010.7188945819272077No Hit
AAGCTGCCAGTTGAAGAACTGTTCATC232730.7089035890509684No Hit
TGAGGTAGTAGTTTGTGCTGTTTCATC213150.6492622352348812No Hit
TGAGAACTGAATTCCATGGGTTTCATC192700.5869708314790598No Hit
TGAGGTAGTAGATTGTATAGTTTCATC190350.579812650607364No Hit
TCCTGTACTGAGCTGCCCCGAGTCATC158100.4815780407723891No Hit
CGTACCGTGAGTAATAATGCGTCATC157080.47847108567063174No Hit
TCAGTGCACTACAGAACTTTGTTCATC145850.44426411920716596No Hit
TTCAAGTAATTCAGGATAGGTTTCATC145120.44204051408532No Hit
GGCTGGTCCGATGGTAGTGGGTTATCANAACTCATC137520.41889065254281427No Hit
AGGTTCCGGATAAGTAAGAGCCTCATC132320.40305127359267884No Hit
AAACCGTTACCATTACTGAGTTCATC124080.377951950025541No Hit
TCAGTGCATCACAGAACTTTGTTCATC123760.37697721901322495No Hit
CTAGACTGAAGCTCCTTGAGGTCATC122670.3736570415025235No Hit
TGATACGGATGTTATACGCAGCTCATC120700.3676563537079529No Hit
CATTGCACTTGTCTCGGTCTGATCATC120170.36604195546880447No Hit
TCGTACCGTGAGTAATAATGCGTCATC115400.3515123713164687No Hit
CAACGGAATCCCAAAAGCAGCTTCATC109960.3349419441070961No Hit
GGCTGGTCCGATGGTAGTGGGTTATCANAACTTCATC108150.3294286218186836No Hit
TAGCAGCACGTAAATATTGGCGTCATC103310.31468581525740363No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATTTCATC95110.2897083330668053No Hit
TTCACAGTGGCTAAGTTCCGCTCATC95100.2896778727226705No Hit
TGAGGTAGTAGGTTGTGTGGTTTCATC93820.2857789486734063No Hit
AAACCGTTACCATTACTGAGTTTCATC92850.2828242952923234No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACTCATC84860.2584864803285574No Hit
TGTCAGTTTGTCAAATACCCCATCATC84670.2579077337899948No Hit
TCCTGTACTGAGCTGCCCCGATCATC82640.251724283930615No Hit
TGAGGTAGTAGTTTGTACAGTTTCATC81820.24922653571155515No Hit
TTCAAGTAATCCAGGATCGGCTTCATC69830.21270458309383886No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAAAACTTTCATC69260.21096834347815097No Hit
TCTTAACCCGGACCAGAAACTATCATC65330.1989974282331447No Hit
CAGTGCAATGTTAAAAGGGCATTCATC64450.1963169179492756No Hit
GTCCCACTCCGTAGATCTGTTCTCATC63450.193270883535788No Hit
TGAGGTAGTAGGTTGTATAGTTCATC62490.19034669049883993No Hit
TGTCAGTTTGTCAAATACCCCTCATC59380.1808735234728935No Hit
CCCCCCACTGCTAAATTTGACTGGCTTATCATC58400.17788840974767564No Hit
AGCAGCATTGTACAGGGCTATGATCATC58040.17679183735882012No Hit
AGCTTATCAGACTGATGTTGACTCATC54900.16722728930046907No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGACCTTCATC52850.1609829187528195No Hit
ATCACATTGCCAGGGATTTCCTCATC52280.15924667913713156No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTCATC51790.15775412227452262No Hit
AACATTCAACGCTGTCGGTGAGTTCATC51150.1558046602498906No Hit
TTATGGTTTGCCTGGGACTGATCATC50950.15519545336719306No Hit
TGGAGAGAAAGGCAGTTCCTGATCATC48990.14922522591675735No Hit
TGAGACCTGAATTCCATGGGTTTCATC48640.1481591138720367No Hit
TCGAGGAGCTCACAGTCTAGTTCATC47990.14617919150326977No Hit
AAGCTGCCAGTTGAAGACCTGTTCATC47840.14572228634124662No Hit
CTAGACTGAAGCTCCTTGAGGATCATC47650.145143539802684No Hit
AAAAGCTGGGTTGAGAGGGCGATCATC45510.13862502615782052No Hit
TGGCTCAGTTCAGCAGGAACAGTCATC45110.1374066123924255No Hit
TTCACAGTGGCTAAGTTCTGCTCATC44260.13481748314096104No Hit
GGCTGGTCCGATGGGAGTGGGTTATCAGAACTCATC43040.13110132115650616No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACTTCATC42620.12982198670284137No Hit
TCCTGTACTGAGCTGCCCCGAGTTCATC42260.12872541431398582No Hit
TGTAAACATCCCCGACTGGAAGCTTCATC40460.12324255236970817No Hit
CACTAGATTGTGAGCTCCTGGATCATC39670.12083618518305295No Hit
GATGTAACGAGTTGGAATGCAATCATC39390.11998329554727644No Hit
TGAGGTAGTAGATTGTATAGTTCATC38690.11785107145783512No Hit
CATCGGTCGAACTTATGTGAAATCATC37190.11328201983760372No Hit
GGCTGGTCCGATGGTAGGGGGTTATCAGAACTCATC36720.11185038366326457No Hit
TGAGGTAGTAGGTTGTGTGGTTCATC35850.10920033372353036No Hit
GGCTGGTCCGATGGGAGTGGGTTATCAGAACTTCATC35470.10804284064640506No Hit
TTCACAGTGGCTAAGTTCCGTCATC35240.1073422527313029No Hit
GGCTGGTCCGATGGTAGGGGGTTATCAGAACTTCATC35200.1072204113547634No Hit
CCCATAAAGTAGAAAGCACTACTCATC35050.10676350619274026No Hit
TTCAAGTAATCCAGGATAGGCTCATC34580.1053318700184011No Hit
TGCGGGGCTAGGGCTAACAGCATCATC33450.10188985113116011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)